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BiomaterialsVolume 27, Issue 31, November 2006, Pages 5357-5367

Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers(Article)

  • aINEB - Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
  • bUniversidade do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal
  • cDepartment of Bioengineering, Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States

Abstract

The effect of surface wettability on fibrinogen adsorption, platelet adhesion and platelet activation was investigated using self-assembled monolayers (SAMs) containing different ratios of longer chain methyl- and shorter chain hydroxyl-terminated alkanethiols (C15CH3 vs. C11OH) on gold. Protein adsorption studies were performed using radiolabeled human fibrinogen (HFG). Platelet adhesion and activation studies with and without pre-adsorbed fibrinogen, albumin and plasma were assessed using scanning electron microscopy (SEM) and a glutaraldehyde-induced fluorescence technique (GIFT). Results demonstrated a linear decrease of HFG adsorption with the increase of OH groups on the monolayer (increase of the hydrophilicity). Platelet adhesion and activation also decrease with increase of hydrophilicity of surface. Concerning SAMs pre-immersed in proteins, fibrinogen adsorption was related with high platelet adhesion and activation. The passivant effect of albumin on platelet adhesion and activation was only demonstrated on SAMs contained C11OH. When all the blood proteins are present (plasma) platelet adhesion was almost absent on SAMs with 65% and 100% C11OH. This could be explained by the higher albumin affinity of the SAMs with 65% C11OH and the lower total protein adsorption associated with SAMs with 100% C11OH. © 2006 Elsevier Ltd. All rights reserved.

Author keywords

FibrinogenPlatelet activationPlatelet adhesionProtein adsorptionSelf assembledSEM

Indexed keywords

Engineering controlled terms:Chemical reactionsFluorescenceHydrophilicityOrganic compoundsProteinsScanning electron microscopy
Engineering uncontrolled terms:FibrinogenPlatelet activationPlatelet adhesionProtein adsorptionRadiolabeled human fibrinogen (HFG)
Engineering main heading:Monolayers
EMTREE drug terms:albuminalkane derivativefibrinogenglutaraldehydegoldhydroxyl groupmethyl groupplasma proteinthiol derivative
EMTREE medical terms:adsorptionarticlebinding affinitycontrolled studyfluorescence analysishumanhuman cellhydrophilicitymonolayer culturenormal humanplasmapriority journalscanning electron microscopysurface propertythrombocyte adhesionwettability
MeSH:AdsorptionAlkanesBiocompatible MaterialsBlood PlateletsCells, CulturedCrystallizationFibrinogenHumansHydroxidesMaterials TestingMethylationPlatelet ActivationPlatelet AdhesivenessProtein BindingSulfhydryl CompoundsWettability

Chemicals and CAS Registry Numbers:

fibrinogen, 9001-32-5; glutaraldehyde, 111-30-8, 37245-61-7; gold, 7440-57-5; thiol derivative, 13940-21-1;

Alkanes; Biocompatible Materials; Fibrinogen, 9001-32-5; hydroxide ion, 14280-30-9; Hydroxides; Sulfhydryl Compounds

Funding details

Funding sponsor Funding number Acronym
Programa Operacional Temático Factores de Competitividade
National Science FoundationEEC-9529161,POCI/CTM/55644/2004
Fuel Cell Technologies ProgramSFRH/BD/188337/2004FCT
Federación Española de Enfermedades RarasFEDER

  • 1

    The authors would like to Portuguese Blood Institute (IPS) for donating the platelet concentrate (PC) and the human plasma, by assistance and by enabling them to carry out the platelet adhesion tests. Inês Gonçalves is grateful to the Portuguese Foundation for Science and Technology (FCT) for awarding her the Grant SFRH/BD/188337/2004. Ma Cristina L. Martins is grateful to the Portuguese Foundation for Science and Technology (FCT) for awarding her a post-doctoral scholarship. Professor Ratner acknowledges support from UWEB (NSF Grant EEC-9529161). This work was carried out under contract POCI/CTM/55644/2004, financed by FEDER, through Programa Operacional Ciência e Inovação 2010.

  • ISSN: 01429612
  • CODEN: BIMAD
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1016/j.biomaterials.2006.06.010
  • PubMed ID: 16842847
  • Document Type: Article

  Martins, M.C.L.; INEB - Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Universidade do Porto, Rua do Campo Alegre, 823, Portugal;
© Copyright 2008 Elsevier B.V., All rights reserved. © MEDLINE® is the source for the MeSH terms of this document.

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