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VirulenceVolume 8, Issue 6, 28 October 2017, Pages 993-1004

Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection(Article)(Open Access)

  • Pinheiro, J.,
  • Reis, O.,
  • Vieira, A.,
  • Moura, I.M.,
  • Moreno, L.Z.,
  • Carvalho, F.,
  • Pucciarelli, M.G.,
  • García-del Portillo, F.,
  • Sousa, S.,
  • Cabanes, D.
  • View Correspondence (jump link)
  • aInstituto de Investigaçao e Inovação em Saúde - i3S, Universidade do Porto, Porto, Portugal
  • bGroup of Molecular Microbiology, Instituto de Biologia Molecular e Celular - IBMC, Porto, Portugal
  • cInstituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal
  • dLaboratório de Saúde Pública, Faculdade de Saude Publica, Universidade de São Paulo, São Paulo, Brazil
  • eCentro Nacional de Biotecnología-CSIC (CNB-CSIC), Madrid, Spain
  • fDepartamento de Biología Molecular, Universidad Autonoma de Madrid, Centro de Biologıa Molecular “Severo Ochoa” (CBMSO-CSIC), Madrid, Spain

Abstract

Bacterial pathogenicity deeply depends on the ability to secrete virulence factors that bind specific targets on host cells and manipulate host responses. The Gram-positive bacterium Listeria monocytogenes is a human foodborne pathogen that remains a serious public health concern. To transport proteins across its cell envelope, this facultative intracellular pathogen engages a set of specialized secretion systems. Here we show that L. monocytogenes EGDe uses a specialized secretion system, named ESX-1, to secrete EsxA, a homolog of the virulence determinants ESAT-6 and EsxA of Mycobacterium tuberculosis and Staphylococcus aureus, respectively. Our data show that the L. monocytogenes ESX-1 secretion system and its substrates are dispensable for bacterial invasion and intracellular multiplication in eukaryotic cell lines. Surprisingly, we found that the EssCdependent secretion of EsxA has a detrimental effect on L. monocytogenes in vivo infection. © 2017 Taylor & Francis.

Author keywords

ESAT-6ESX-1 secretion systemEsxAListeria monocytogenesType VII secretion system

Indexed keywords

EMTREE drug terms:virulence factorbacterial antigenbacterial proteinESAT-6 protein, Mycobacterium tuberculosis
EMTREE medical terms:animal experimentanimal modelanimal tissueArticlebacterial countbacterial growthbacterial virulencebioinformaticscell invasion assaycontrolled studyDNA sequencefemalegene expressiongene overexpressiongenetic complementationgrowth curveimmune responseListeria monocytogeneslisteriosismouseMycobacterium tuberculosisnonhumanpolyacrylamide gel electrophoresisreverse transcription polymerase chain reactionsequence homologyStaphylococcus aureustype VII secretion systemWestern blottingwhole genome sequencingbacterial secretion systemcell linegeneticsgrowth, development and aginghumanmetabolismpathogenicity
MeSH:Antigens, BacterialBacterial ProteinsBacterial Secretion SystemsCell LineHumansListeria monocytogenesMycobacterium tuberculosisStaphylococcus aureusVirulence Factors

Chemicals and CAS Registry Numbers:

Antigens, Bacterial; Bacterial Proteins; Bacterial Secretion Systems; ESAT-6 protein, Mycobacterium tuberculosis; Virulence Factors

Funding details

Funding sponsor Funding number Acronym
Programa Operacional Temático Factores de CompetitividadeBIO2013-46281-P,BIO2014-55238-R
Federación Española de Enfermedades RarasInfect-ERA/0001/2013 PROANTILIS,SFRH/BD/61825/ 2009,4293,SFRH/BD/ 28185/2006,SFRH/BD/86871/2012FEDER

  • 1

    This work was supported for the DC lab by national funds through FCT - Funda?ao para a Ciencia e a Tecnologia/MEC - Ministerio da Educa?ao e Ciencia and co-funded by FEDER funds within the partnership agreement PT2020 related with the research unit number 4293, and within the research project Infect-ERA/0001/2013 PROANTILIS. O.R., J.P. and F.C. were supported by doctoral fellowships from FCT (SFRH/BD/ 28185/2006, SFRH/BD/86871/2012 and SFRH/BD/61825/ 2009), L.M. by the Santander International Mobility Program and SS by FCT Investigator program (COMPETE, POPH, and FCT). This work was supported for the F.G.-dP. lab by grants BIO2014-55238-R (to M.G.P.) and BIO2013-46281-P (to F.G.-dP.) from the Spanish Ministry of Economy and Competitiveness.

  • ISSN: 21505594
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1080/21505594.2016.1244589
  • PubMed ID: 27723420
  • Document Type: Article
  • Publisher: Taylor and Francis Inc.

  Cabanes, D.; I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto IBMC - Institute for Molecular and Cell Biology, GM2 - Group of Molecular Microbiology, Rua Alfredo Allen, 208, Porto, Portugal;
© Copyright 2018 Elsevier B.V., All rights reserved.

Cited by 6 documents

Wang, Y. , Li, X. , Osmundson, T.
Comparative Genomic Analysis of a Multidrug-Resistant Listeria monocytogenes ST477 Isolate
(2019) Foodborne Pathogens and Disease
Tiwari, S. , Casey, R. , Goulding, C.W.
Inject and infect: How Mycobacterium tuberculosis exploits its major virulence-associated type VII secretion system, ESX-1
(2019) Microbiology Spectrum
Hara, H. , Seregin, S.S. , Yang, D.
The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection
(2018) Cell
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