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NanomedicineVolume 11, Issue 24, December 2016, Pages 3205-3221

Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles(Article)

  • aINEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, Porto, 4200-135, Portugal
  • bI3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, Porto, 4200-135, Portugal
  • cFaculdade de Medicina, Universidade do Porto, Alameda Prof Hernâni Monteiro, Porto, 4200-319, Portugal
  • dFaculdade de Engenharia, Universidade do Porto, Rua Dr Roberto Frias, s/n, Porto, 4200-465, Portugal
  • eInstituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen, 208, Porto, 4200-135, Portugal
  • fInstituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, 4050-313, Portugal

Abstract

Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance. © 2016 Future Medicine Ltd.

Author keywords

gene deliverymicrofluidicstargeted nanoparticles

Indexed keywords

EMTREE drug terms:chitosan nanoparticleneurotropic agenttrimethyl chitosan nanoparticleunclassified drugchitosanimmunoglobulin heavy chainnanoparticle
EMTREE medical terms:animal cellArticlebiological activitycell compartmentalizationcontrolled studyembryogene targetingin vitro studyin vivo studyinternalizationmicrofluidicsnanopharmaceuticsnerve cellnerve cell culturenerve endingnonhumanperikaryonperipheral nervous systempriority journalquantitative analysisratretrograde nerve fiber transportspinal gangliontoxicity testingtransport kineticsadministration and dosageanimalaxoncell culturechemistrycytologydrug effectsgene therapygene transfergeneticsmammalian embryometabolismmicrofluidicsnerve cellplasmidproceduresWistar rat
MeSH:AnimalsAxonsCells, CulturedChitosanEmbryo, MammalianGene Transfer TechniquesGenetic TherapyImmunoglobulin Heavy ChainsMicrofluidicsNanoparticlesNeuronsPlasmidsRatsRats, Wistar

Chemicals and CAS Registry Numbers:

chitosan, 9012-76-4;

Chitosan; Immunoglobulin Heavy Chains

  • ISSN: 17435889
  • Source Type: Journal
  • Original language: English
  • DOI: 10.2217/nnm-2016-0247
  • PubMed ID: 27830593
  • Document Type: Article
  • Publisher: Future Medicine Ltd.

  Pêgo, A.P.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, Porto, Portugal;
© Copyright 2017 Elsevier B.V., All rights reserved.

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