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Sensors (Switzerland)Volume 17, Issue 1, January 2017, Article number 34

Organophosphorous pesticide detection in olive oil by using a miniaturized, easy-to-use, and cost-effective biosensor combined with quechers for sample clean-up(Article)(Open Access)

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  • aDepartment of Chemical Science and Technologies, University of Rome ‘Tor Vergata’, Via della Ricerca Scientifica, Rome, 00133, Italy
  • bInstitute of Crystallography (IC-CNR), Via Salaria km 29.300, Monterotondo, 00015, Italy
  • cDepartment of Analytical Chemistry, Institute of Chemical Engineering, Ural Federal University Named After the First President of Russia B. N. Yeltsin, Mira 19, Yekaterinburg, 620002, Russian Federation

Abstract

Herein, we report a portable electrochemical biosensor based on butyrylcholinesterase (BChE) immobilized on carbon black (CB)-modified screen-printed electrodes (SPEs) for the detection of organophosphorous pesticides in olive oil. The BChE/CB-SPE biosensor was developed to detect paraoxon in standard solutions as well as in olive oil samples previously treated with the QuEChERS method to extract pesticides from the whole fatty matrix. The biosensor shows a linear concentration range of between 20 and 100 ppb for paraoxon both in standard solutions (phosphate buffer 0.05 M) and in olive oil extracts, with a detection limit of 6 ppb in olive oil extract, corresponding to 10% of inhibition. The accuracy of this biosensor in olive oil samples was assessed with olive oil spiked with paraoxon, obtaining satisfactory recovery values. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

Author keywords

ButyrylcholinesteraseCarbon blackElectrochemical biosensorOrganophosphorous pesticidesQuEChERSScreen-printed electrodes

Indexed keywords

Engineering controlled terms:BiosensorsCarbon blackCost effectivenessElectrochemical electrodesPesticides
Engineering uncontrolled termsButyrylcholinesteraseElectrochemical biosensorOrganophosphorous pesticidesQuechersScreen printed electrodes
Engineering main heading:Olive oil
EMTREE drug terms:olive oilorganophosphorus compoundpesticide
EMTREE medical terms:chemistryelectrochemistrygenetic procedureslimit of detectionprocedures
MeSH:Biosensing TechniquesElectrochemistryLimit of DetectionOlive OilOrganophosphorus CompoundsPesticides

Chemicals and CAS Registry Numbers:

olive oil, 8001-25-0;

Olive Oil; Organophosphorus Compounds; Pesticides

  • ISSN: 14248220
  • Source Type: Journal
  • Original language: English
  • DOI: 10.3390/s17010034
  • PubMed ID: 28029127
  • Document Type: Article
  • Publisher: MDPI AG

  Arduini, F.; Department of Chemical Science and Technologies, University of Rome ‘Tor Vergata’, Via della Ricerca Scientifica, Rome, Italy;
© Copyright 2021 Elsevier B.V., All rights reserved.

Cited by 12 documents

Soldatkin, O.O. , Pyeshkova, V.M. , Kucherenko, I.S.
Application of butyrylcholinesterase-based biosensor for simultaneous determination of different toxicants using inhibition and reactivation steps
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Marx, Í.M.G.
Emerging Trends of Electrochemical Sensors in Food Analysis
(2023) Electrochem
Poudyal, D.C. , Dhamu, V.N. , Samson, M.
Pesticide analytical screening system (PASS): A novel electrochemical system for multiplex screening of glyphosate and chlorpyrifos in high-fat and low-fat food matrices
(2023) Food Chemistry
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