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Open BiologyVolume 7, Issue 1, 2017, Article number 160258

TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands(Article)(Open Access)

  • aI3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, 4150-180, Portugal
  • bInstituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Porto, 4150-180, Portugal
  • cCell Cycle Development Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, United Kingdom
  • dCentro Andaluz de Biología del Desarrollo (CABD), CSIC-JA, Universidad Pablo de Olavide, Ctra. de Utrera km1, Seville, 41013, Spain

Abstract

Signalling by TGFb superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFb/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFb/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFb/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis. © 2017 The Authors.

Author keywords

Cell growthDrosophilaNucleolusPunt (Put)RibosomeTGFb/Activin

Indexed keywords

EMTREE drug terms:activinactivin receptor 2Drosophila proteinput protein, DrosophilaSmad2 proteinSmad2 protein, Drosophilatransforming growth factor beta
EMTREE medical terms:animalcell cyclecell proliferationDrosophila melanogasterembryologygene expression regulationgene silencinggeneticsgrowth, development and agingmetabolismnucleolusribosomesalivary glandsignal transduction
MeSH:Activin Receptors, Type IIActivinsAnimalsCell CycleCell NucleolusCell ProliferationDrosophila melanogasterDrosophila ProteinsGene Expression Regulation, DevelopmentalGene Knockdown TechniquesRibosomesSalivary GlandsSignal TransductionSmad2 ProteinTransforming Growth Factor beta

Chemicals and CAS Registry Numbers:

activin, 114949-22-3; Smad2 protein, 253862-89-4;

Activin Receptors, Type II; Activins; Drosophila Proteins; put protein, Drosophila; Smad2 Protein; Smad2 protein, Drosophila; Transforming Growth Factor beta

Funding details

Funding sponsor Funding number Acronym
Fundação Portugal Telecom
China National Funds for Distinguished Young Scientists
Fundação para a Ciência e a Tecnologia
See opportunities
SFRH/BD/95087/2013,FCOMP-01-0124-FEDER-015675,PTDC/SAU-BID/112250/2009,ALTF 677-2012
European Regional Development Fund
NORTE 2020

  • 1

    This work was supported by project Norte-01-0145-FEDER-000029, Advancing Cancer Research: From Basic Knowledge to Application, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and by National Funds through FCT-'Funda??o para a Ci?ncia e a Tecnologia' under the project FCOMP-01-0124-FEDER-015675 (PTDC/SAU-BID/112250/2009). T.M. was supported by an FCT Fellowship ALTF 677-2012, and N.E. was supported by a PhD FCT Fellowship SFRH/BD/95087/2013. P.S.P. is a recipient of a Portuguese 'Investigator FCT' contract.

  • ISSN: 20462441
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1098/rsob.160258
  • PubMed ID: 28123053
  • Document Type: Article
  • Publisher: Royal Society of London

  Martins, T.; I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal;
© Copyright 2017 Elsevier B.V., All rights reserved.

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