Document details
Novel sintering-free scaffolds obtained by additive manufacturing for concurrent bone regeneration and drug delivery: Proof of concept(Article)
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- aDepartment of Materials and Ceramics Engineering, CICECO University of Aveiro, Aveiro, 3810-193, Portugal
- bi3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
- cINEB – Instituto Nacional de Engenharia Biomédica, 4200-135, Portugal
- dUIDM, ESTG, Polytechnic Institute of Viana do Castelo, Viana do Castelo, 4900, Portugal
- eDepartment of Chemistry-CICECO, University of Aveiro, Aveiro, 3810-193, Portugal
- fResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Lisbon, 1649-003, Portugal
- gFEUP – Faculdade de Engenharia da Universidade do Porto, 4200-464, Portugal
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Abstract
Advances on the fabrication of sintering-free biphasic calcium phosphate (BCP)/natural polymer composite scaffolds using robocasting as additive manufacturing technique are presented in the present work. Inks with high amounts of BCP powders (45 vol%) containing different HA/β-TCP ratios, in presence of crosslinked polymer, were successfully fine-tuned for extrusion by robocasting. The non-existence of sintering step opened the possibility to obtain drug loaded scaffolds by adding levofloxacin to the extrudable inks. The drug presence induced slightly changes on the rheological behaviour of the inks, more emphasized for the BCP compositions with higher amounts of β-TCP, and consequently, on the microstructure and on the mechanical properties of the final scaffolds. The strong interaction of β-TCP with chitosan difficult the preparation of suitable rheological inks for printing. Drug delivery studies revealed a fast release of levofloxacin with a high burst of drug within the first 30 min. Levofloxacin loaded samples also presented bacteria growth inhibition ability, proving that antibiotic was not degraded during the fabrication process and its bactericidal efficacy was preserved. From the results obtained, the composite scaffolds containing higher amounts of HA (around 80% HA/20% β-TCP) constitute a promising bi-functional synthetic bone substitute for simultaneous local bone regeneration and infection treatments. © 2018
Indexed keywords
| Engineering controlled terms: | 3D printersBoneCalcium phosphateControlled drug deliveryScaffolds (biology)SinteringTransmission control protocol |
|---|---|
| Engineering uncontrolled terms | Biphasic calcium phosphatesBone regenerationComposite scaffoldsCross-linked polymersLocal drug deliveryManufacturing techniquesRheological behaviourRobocasting |
| Engineering main heading: | Targeted drug delivery |
| EMTREE drug terms: | levofloxacin |
| EMTREE medical terms: | bone regenerationcell countcell deathchemistrycytologydrug delivery systemdrug effectdrug releasefibroblasthumanmicrobial sensitivity testnewbornphysiologypowderRaman spectrometryStaphylococcus aureustemperaturethree dimensional imagingtissue scaffoldviscosityYoung modulus |
| MeSH: | Bone RegenerationCell CountCell DeathDrug Delivery SystemsDrug LiberationElastic ModulusFibroblastsHumansImaging, Three-DimensionalInfant, NewbornLevofloxacinMicrobial Sensitivity TestsPowdersSpectrum Analysis, RamanStaphylococcus aureusTemperatureTissue ScaffoldsViscosity |
Chemicals and CAS Registry Numbers:
levofloxacin, 100986-85-4, 138199-71-0;
Levofloxacin; Powders
Funding details
| Funding sponsor | Funding number | Acronym |
|---|---|---|
| Fundação Portugal Telecom | ||
| Fundação para a Ciência e a Tecnologia See opportunities | ||
| POCI/COMPETE 2020/FEDER | ||
| UID/CTM/50011/2013,POCI-01-0145-FEDER-007679 | ||
| SFRH/BD/78355/2011 | ||
| Universidade de Aveiro | 2BBone,POCI-01-0145-FEDER-029940 | |
| European Regional Development Fund | 2020,NORTE 2020 |
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1
This study was financially supported by the projects of CICECO-Aveiro Institute of Materials, POCI-01-0145-FEDER-007679 (Ref. UID/CTM/50011/2013) University of Aveiro, and 2BBone (POCI-01-0145-FEDER-029940), financed by Operational Programme for Competitiveness and Internationalization (POCI/COMPETE 2020/FEDER) and national funds through the Portuguese Foundation for Science and Technology (FCT/MEC). C.F. Marques is grateful for the Grant SFRH/BD/78355/2011 from FCT. S.Olhero project IF/00951/2014 from FCT is also acknowledged. P.L. Granja and A. Sousa are grateful to funding by European Regional Development Fund (ERDF) through the COMPETE 2020-POCI, Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, and by Portuguese funds through FCT. iMED.ULisboa projects provided by FCT through Pest-UID/DTP/04138/2014 are also acknowledged. Authors would like to thank Micronsense - Metrologia Industrial (Leiria, Portugal) for the μCT analysis.
- ISSN: 09284931
- Source Type: Journal
- Original language: English
- DOI: 10.1016/j.msec.2018.09.050
- PubMed ID: 30423726
- Document Type: Article
- Publisher: Elsevier Ltd
Marques, C.F.; Department of Materials and Ceramics Engineering, CICECO University of Aveiro, Aveiro, Portugal;
© Copyright 2018 Elsevier B.V., All rights reserved.
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