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Nucleic Acids ResearchVolume 46, Issue 18, 2018, Pages 9338-9352

MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence(Article)(Open Access)

  • Pinheiro, J.,
  • Lisboa, J.,
  • Pombinho, R.,
  • Carvalho, F.,
  • Carreaux, A.,
  • Brito, C.,
  • Pöntinen, A.,
  • Korkeala, H.,
  • Dos Santos, N.M.S.,
  • Morais-Cabral, J.H.,
  • Sousa, S.,
  • Cabanes, D.
  • View Correspondence (jump link)
  • aGroup of Molecular Microbiology, IBMC-Institute for Molecular and Cell Biology, i3S-Institute for Research and Innovation in Health, Porto, 4200-135, Portugal
  • bICBAS-Instituto de Ciencias Biomédicas Abel Salazar, University of Porto, Porto, 4200-135, Portugal
  • cGroup of Fish Immunology and Vaccinology, IBMC-Institute for Molecular and Cell Biology, i3S-Institute for Research and Innovation in Health, Porto, 4200-135, Portugal
  • dSDV-UFR Sciences du Vivant, Université Paris Diderot-Paris 7, Paris, 75013, France
  • eDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, 00014, Finland
  • fGroup of Structural Biochemistry, IBMC-Institute for Molecular and Cell Biology, I3S-Institute for Research and Innovation in Health, Porto, 4200-135, Portugal

Abstract

The foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible animals and humans. To survive and proliferate within hosts, this facultative intracellular pathogen tightly coordinates the expression of a complex regulatory network that controls the expression of virulence factors. Here, we identified and characterized MouR, a novel virulence regulator of Lm. Through RNA-seq transcriptomic analysis, we determined the MouR regulon and demonstrated how MouR positively controls the expression of the Agr quorum sensing system (agrBDCA) of Lm. The MouR three-dimensional structure revealed a dimeric DNA-binding transcription factor belonging to the VanR class of the GntR superfamily of regulatory proteins. We also showed that by directly binding to the agr promoter region, MouR ultimately modulates chitinase activity and biofilm formation. Importantly, we demonstrated by in vitro cell invasion assays and in vivo mice infections the role of MouR in Lm virulence. © 2018 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.

Indexed keywords

EMTREE drug terms:chitinaseprotein MouRunclassified drugvirulence factorbacterial proteinbacterial RNARNAIII, Staphylococcus aureustranscription factorvirulence factor
EMTREE medical terms:agrBDCA geneanimal experimentanimal modelanimal tissueArticlebacterial genebacterial virulencebiofilmcell invasioncontrolled studyenzyme activityfemalehumanhuman cellimmune evasionin vitro studyin vivo studyListeria monocytogeneslisteriosismousenonhumanpriority journalpromoter regionquorum sensingregulonRNA sequencetranscriptomicsgene expression profilinggene expression regulationgeneticsListeria monocytogenesmetabolismpathogenicityphysiologysite directed mutagenesistransgenic organismvirulence
MeSH:Bacterial ProteinsGene Expression ProfilingGene Expression Regulation, BacterialListeria monocytogenesMutagenesis, Site-DirectedOrganisms, Genetically ModifiedRegulonRNA, BacterialTranscription FactorsVirulenceVirulence Factors

Chemicals and CAS Registry Numbers:

chitinase, 9001-06-3;

Bacterial Proteins; RNA, Bacterial; RNAIII, Staphylococcus aureus; Transcription Factors; Virulence Factors

Funding details

Funding sponsor Funding number Acronym
Fundació Catalana de TrasplantamentFCT

  • 1

    Norte-01-0145-FEDER-000012-Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by Norte Portugal Regional Operational Programme [NORTE 2020], under the Portugal Partnership Agreement, through the European Regional Development Fund (FEDER); FCT Doctoral Fellowship [SFRH/BD/86871/2012, SFRH/BD/89542/2012, SFRH/BD/61825/2009 and SFRH/BD/112217/2015 to J.P., R.P., F.C. and C.B.] through FCT/MEC co-funded by QREN and POPH (Programa Operacional Potencial Humano); FCT Investigator program (COMPETE, POPH and FCT) (to S.S.). Funding for open access charge: Norte-01-0145-FEDER-000012-Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by Norte Portugal Regional Operational Programme [NORTE 2020].

  • ISSN: 03051048
  • CODEN: NARHA
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1093/nar/gky624
  • PubMed ID: 30011022
  • Document Type: Article
  • Publisher: Oxford University Press

  Cabanes, D.; Group of Molecular Microbiology, IBMC-Institute for Molecular and Cell Biology, I3S-Institute for Research and Innovation in Health, Porto, Portugal;
© Copyright 2019 Elsevier B.V., All rights reserved.

Cited by 2 documents

Iacob, S. , Iacob, D.G. , Luminos, L.M.
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(2019) Frontiers in Microbiology
Dorey, A. , Marinho, C. , Piveteau, P.
Role and regulation of the stress activated sigma factor sigma B (σ B ) in the saprophytic and host-associated life stages of Listeria monocytogenes
(2019) Advances in Applied Microbiology
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