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Materials HorizonsVolume 5, Issue 6, November 2018, Pages 1100-1111

A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering(Article)

  • aI3S-Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135, Portugal
  • bINEB-Instituto Nacional de Engenharia Biomédica, University of Porto, 4200-135, Portugal
  • cICBAS-Instituto de Ciências Biomédicas Abel Salazar, University of Porto, 4050-343, Portugal
  • dCDRSP-Centre for Rapid and Sustainable Product Development, Polytechnic Institute of Leiria, 2430-028, Portugal
  • eSchool of Mechanical, Aerospace and Civil Engineering, University of Manchester, M13 9PL, United Kingdom
  • fManchester Institute of Biotechnology, University of Manchester, M1 7DN, United Kingdom

Abstract

Bioprinting is attractive to create cellularized constructs for skin repair. However, the vast majority of bioinks present limitations in the printing of chemically defined 3D constructs with controllable biophysical and biochemical properties. To address this challenge, a single-component hydrogel bioink with a controlled density of cell-adhesive ligands, tuneable mechanical properties and adjustable rheological behaviour is developed for extrusion bioprinting and applied for the biofabrication of 3D dermal constructs. A methacrylate modified pectin bioink is designed to allow the tethering of integrin-binding motifs and the formation of hydrogels by UV photopolymerization and ionic gelation. The rheological behaviour of a low polymer concentration (1.5 wt%) solution is adjusted by ionic crosslinking, yielding a printable bioink that holds the predesigned shape upon deposition for postprinting photocrosslinking. Printed constructs provide a suitable microenvironment that supports the deposition of endogenous extracellular matrix, rich in collagen and fibronectin, by entrapped dermal fibroblasts. This approach enables the design of chemically defined and cell-responsive bioinks for tissue engineering applications and particularly for the generation of biomimetic skin constructs. © 2018 The Royal Society of Chemistry.

Indexed keywords

Engineering controlled terms:AdhesivesBiomechanicsBiomimeticsCell cultureCell engineeringCrosslinkingDensity (specific gravity)DepositionEnzyme activityGelationHydrogelsPhotopolymerizationTissue
Engineering uncontrolled termsBiochemical propertiesExtracellular matricesIntegrin-binding motifsIonic crosslinkingPolymer concentrationsRheological behaviourTissue engineering applicationsUV photopolymerization
Engineering main heading:Tissue engineering

Funding details

Funding sponsor Funding number Acronym
Fundação Portugal Telecom
Office of Electricity Delivery and Energy ReliabilitySFRH/BPD/90047/2012,SFRH/ BD/91151/2012
Fuel Cell Technologies Program
IF/00296/2015
European Regional Development FundP2020-PTDC/BBB-ECT/2145/2014
NORTE 2020

  • 1

    This work was supported by the project Norte-01-0145-FEDER-000012 – Structured program on bioengineered therapies for infectious diseases and tissue regeneration, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and supported by the project P2020-PTDC/BBB-ECT/2145/2014 funded by POCI via FEDER and by Foundation for Science and Technology (FCT) via OE. R. P., A. S. and C. B. thank FCT for the doctoral grant SFRH/ BD/91151/2012, the post-doctoral grant SFRH/BPD/90047/2012 and the FCT Investigator research position IF/00296/2015 (FCT and POPH/ESF), respectively. The authors thank D. S. and M. A. from CEMUP (Centro de Materiais da Universidade do Porto) for the CryoSEM and 1H NMR analyses, respectively.

  • ISSN: 20516347
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1039/c8mh00525g
  • Document Type: Article
  • Publisher: Royal Society of Chemistry

  Bártolo, P.J.; School of Mechanical, Aerospace and Civil Engineering, University of Manchester, United Kingdom;
© Copyright 2018 Elsevier B.V., All rights reserved.

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