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News of the National Academy of Sciences of the Republic of Kazakhstan, Series of Geology and Technical SciencesVolume 3, Issue 441, 2020, Pages 161-169

Microencapsulation of proteolytic enzymes for industrial application(Article)(Open Access)

  • Kudryashov, L.S.,
  • Uzakov, Y.M.,
  • Tikhonov, S.L.,
  • Tikhonova, N.V.,
  • Diachkova, A.V.
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  • aV. M. Gorbatov Federal Research Center for Food Systems of RAS, Moscow, Russian Federation
  • bAlmaty Technological University, Almaty, Kazakhstan
  • cDepartment of food engineering, Ural state economic University, Ekaterinburg, Russian Federation
  • dUral Federal Universitynamed after the first president of Russia B. N. Eltsin, Ekaterinburg, Russian Federation

Abstract

The aim of the present study was to develop the technology of microencapsulation of proteolytic enzymes in the pseudo-boiling layer which based on the diffusion of maltodextrin into an enzyme, which allows maintaining high enzyme activity for a long time in meat products. It was found that maltodextrin provides high strength capsule walls their integrity during long-term storage. It was shown that the maximum activity of the proteolytic enzyme pepsin immobilized in a maltodextrin matrix shifts to the alkaline side with increasing pH of the reaction medium, which is not typical for a free enzyme. The results of determining the cutoff voltage of ham from pork ham confirmed that the immobilized enzyme remained active for 6 months of storage. While pure pepsin after 3 months of storage noticeably lost its proteolytic effect. Considering the results obtained microencapsulation of the proteolytic enzyme pepsin using maltodextrin with a coating thickness of 4 to 6 μm can be recommended which will expand the possibilities of using enzymes in the production of meat products. © 2020, National Academy of Sciences of the Republic of Kazakhstan. All rights reserved.

Author keywords

ActivityHam productsImmobilized enzymeMaltodextrinMicroencapsulationPepsin
  • ISSN: 22245278
  • Source Type: Journal
  • Original language: English
  • DOI: 10.32014/2020.2518-170X.67
  • Document Type: Article
  • Publisher: National Academy of Sciences of the Republic of Kazakhstan


© Copyright 2020 Elsevier B.V., All rights reserved.

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