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ChromatographiaVolume 45, 1997, Pages 145-148

Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography(Conference Paper)

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  • aDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Vojvode Stepe 450, Belgrade 11000, Serbia
  • bTorlak-Institute of Immunology and Virology, Vojvode Stepe 458, Belgrade 11221, Serbia

Abstract

TLC and HPLC methods for the determination of the preservative, 8- hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid: 2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.

Author keywords

8-Hydroxyquinoline sulfate-preservativeColumn liquid chromatographyPPD-tuberculin solutionThin-layer chromatography

Indexed keywords

EMTREE drug terms:8 quinolinol sulfateformic acidphosphoric acidtuberculin
EMTREE medical terms:accuracyconference paperdensitometryhigh performance liquid chromatographypriority journalreproducibilitythin layer chromatography

Chemicals and CAS Registry Numbers:

8 quinolinol sulfate, 134-31-6, 14534-95-3, 3819-18-9; formic acid, 64-18-6, 71-47-6; phosphoric acid, 7664-38-2; tuberculin, 92129-86-7

  • ISSN: 00095893
  • CODEN: CHRGB
  • Source Type: Journal
  • Original language: English
  • DOI: 10.1007/BF02505552
  • Document Type: Conference Paper
  • Publisher: Friedr. Vieweg und Sohn Verlags GmbH

  Agbaba, D.; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Vojvode Stepe 450, Serbia
© Copyright 2019 Elsevier B.V., All rights reserved.

Cited by 3 documents

Pushpanjali, P.A. , Manjunatha, J.G.
A sensitive novel approach towards the detection of 8-hydroxyquinoline at anionic surfactant modified carbon nanotube based biosensor: A voltammetric study
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A glutathione derivative with chelating and in vitro neuroprotective activities: Synthesis, physicochemical properties, and biological evaluation
(2013) ChemMedChem
Mihajlović, A. , Agbaba, D. , Živanov-Stakić, D.
High-performance liquid chromatographic assay of 8-hydroxyquinoline sulfate and its stability in immunobiological preparations
(1998) Journal of Chromatography A
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