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A specific and simple HPLC procedure was developed, su table for the determination of dindamycin hydrochloride monohydrate (CLHC) and clindamycin 2-dihydrogenphosphate (CLPH) in pharmaceutical formulations. Rp-HPLC analysis was performed with a LCD-Analytical Consta Metric 3000 system, equipped with a Spectro Monitor 3100 UV/VIS detector. Lincosamides assay procedures were carried out on a Supelcosil^R LC-18-DB (250 × 4,6 mm, 5 μm particles) column in an acetonitrile-potassium dihydrogenphosphate buffer (0.1 M; pH 2.50) (225:775 v/v) as mobile phase. Column effluents were monitored at 206 nm for clindamycin. The calibration curves in concentration ranges from 200 to 1800 μg/ml were established for the investigated clindamycins. The HPLC method was applied for the determination of clindamycin in pharmaceutical formulations. The obtained results and statistical parameters for both the investigated clindamycins in pharmaceutical formulations, were satisfactory and ranged from 99.99% to 104.18% (R.S.D. was ranged from 0.79% to 0.99%).