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A simple HPLC procedure, suitable for the determination of sodium fusidate (SF) in dosage forms, was developed. The method involves using a UV detector (208 nm) in the presence of basic buffered media (pH 7.25) under reversed-phase conditions (Supelcosil® LC18™, 3 μm particles). The linear regression between the concentration of SF and the peak area, γ = -4.4566 + 8.9238x with a coefficient of correlation r = 0.9985, was confirmed for SF in the concentration range from 25 μg/ml to 150 μg/ml. The detection limit of SF was 12.5 μg/ml. The HPLC method was applied for the determination of SF in dosage forms: Stanicid® coated tablets, Stanicid® ointment and Stanicid® sterile gauze dressing. The concentrations and statistical parameters of sodium fusidate for all the investigated dosage forms were satisfactory and ranged from 100.41 to 105.39% (the R. S. D. ranged from 1.51 to 2.75%).