Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments(Article)
- aFaculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Trg Dositeja Obradovića 3, Novi Sad, 21000, Serbia
- bDepartment of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, Bratislava, 845 51, Slovakia
- cFaculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia
- dEnvironmental Microbiology Laboratory, Ecole Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland
Abstract
Investigation of bacterial chromate tolerance has mostly focused on strains originating from polluted sites. In the present study, we isolated 33 chromate tolerant strains from diverse environments harbouring varying concentrations of chromium (Cr). All of these strains were able to grow on minimal media with at least 2 mM hexavalent chromium (Cr(VI)) and their classification revealed that they belonged to 12 different species and 8 genera, with a majority (n = 20) being affiliated to the Bacillus cereus group. Selected B. cereus group strains were further characterised for their chromate tolerance level and the ability to remove toxic Cr(VI) from solution. A similar level of chromate tolerance was observed in isolates originating from environments harbouring high or low Cr. Reference B. cereus strains exhibited the same Cr(VI) tolerance which indicates that a high chromate tolerance could be an intrinsic group characteristic. Cr(VI) removal varied from 22.9% (strain PCr2a) to 98.5% (strain NCr4). Strains NCr1a and PCr12 exhibited the ability to grow to the greatest extent in Cr(VI) containing media (maximum growth of 65.3% and 64.9% relative to that in the absence of Cr(VI), respectively) accompanied with high chromate removal activity (73.7% and 74.4%, respectively), making them prime candidates for the investigation of chromate tolerance mechanisms in Gram-positive bacteria and Cr(VI) bioremediation applications. © 2019, Springer Nature B.V.
Indexed keywords
| Engineering controlled terms: | Bacillus cereusBacteriologyBioremediationChromatesNitrogen compoundsSerpentineSilicate minerals |
|---|---|
| Engineering uncontrolled terms | Bacillus cereus groupsCultivable bacteriaHexavalent chromium removalsSerpentine soilsUnpolluted |
| Engineering main heading: | Phosphorus compounds |
| EMTREE drug terms: | chromic acidchromiumchromium hexavalent ionRNA 16S |
| EMTREE medical terms: | Bacillusbacteriumbioremediationchemistryclassificationculture mediumdrug effectdrug toleranceenvironmental microbiologygeneticsisolation and purificationmicrobial sensitivity testmicrobiologyoxidation reduction reactionphylogenysedimentsoil pollutant |
| MeSH: | BacillusBacteriaBiodegradation, EnvironmentalChromatesChromiumCulture MediaDrug ToleranceEnvironmental MicrobiologyGeologic SedimentsMicrobial Sensitivity TestsOxidation-ReductionPhylogenyRNA, Ribosomal, 16SSoil MicrobiologySoil Pollutants |
Chemicals and CAS Registry Numbers:
chromic acid, 11104-59-9; chromium, 16065-83-1, 7440-47-3, 14092-98-9;
Chromates; Chromium; chromium hexavalent ion; Culture Media; RNA, Ribosomal, 16S; Soil Pollutants
Funding details
| Funding sponsor | Funding number | Acronym |
|---|---|---|
| Agentúra na Podporu Výskumu a Vývoja | APVV | |
| Direktion für Entwicklung und Zusammenarbeit | SCOPES IZ73Z0_152527/1,APVV-14-0181 | DEZA |
| ITMS 26230120006 | ||
| Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung See opportunities by SNF | 152527 | SNF |
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1
Authors would like to thank Albert Breier and Petra Olejnikova from STU Bratislava for MALDI TOF analysis. The project was financed by the SCOPES programme of the Swiss National Science Foundation (SNSF) and the Swiss Agency for Development and Cooperation (SDC) (Grant Number SCOPES IZ73Z0_152527/1) to RBL, DR and IB, and by grant APVV-14-0181 to IB and by grant from Slovak Research Agency (ITMS 26230120006).
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2
Acknowledgements Authors would like to thank Albert Breier and Petra Olejnikova from STU Bratislava for MALDI TOF analysis. The project was financed by the SCOPES programme of the Swiss National Science Foundation (SNSF) and the Swiss Agency for Development and Cooperation (SDC) (Grant Number SCOPES IZ73Z0_152527/1) to RBL, DR and IB, and by grant APVV-14-0181 to IB and by grant from Slovak Research Agency (ITMS 26230120006).
- ISSN: 09593993
- CODEN: WJMBE
- Source Type: Journal
- Original language: English
- DOI: 10.1007/s11274-019-2638-5
- PubMed ID: 30900044
- Document Type: Article
- Publisher: Springer Netherlands
Radnović, D.; Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, Serbia;
© Copyright 2019 Elsevier B.V., All rights reserved.
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