Layer-by-layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo(Article)
- aBiotis, INSERM U1026, Université de Bordeaux, Bordeaux Cedex, France
- bCHU de Bordeaux, Rue de la Pelouse, Bordeaux, France
- cFaculty of Technical Sciences, University of Novi Sad, Novi Sad, Serbia
Abstract
Layer-by-layer (LBL) BioAssembly method was developed to enhance the control of cell distribution within 3D scaffolds for tissue engineering applications. The objective of this study was to evaluate in vivo the development of blood vessels within LBL bioassembled membranes seeded with human primary cells, and to compare it to cellularized massive scaffolds. Poly(lactic) acid (PLA) membranes fabricated by fused deposition modeling were seeded with monocultures of human bone marrow stromal cells or with cocultures of these cells and endothelial progenitor cells. Then, four cellularized membranes were assembled in LBL constructs. Early osteoblastic and endothelial cell differentiation markers, alkaline phosphatase, and von Willebrand's factor, were expressed in all layers of assemblies in homogenous manner. The same kind of LBL assemblies as well as cellularized massive scaffolds was implanted subcutaneously in mice. Human cells were observed in all scaffolds seeded with cells, but not in the inner parts of massive scaffolds. There were significantly more blood vessels observed in LBL bioassemblies seeded with cocultures compared to all other samples. LBL bioassembly of PLA membranes seeded with a coculture of human cells is an efficient method to obtain homogenous cell distribution and blood vessel formation within the entire volume of a 3D composite scaffold. © 2019 Wiley Periodicals, Inc.
Indexed keywords
| Engineering controlled terms: | BloodBlood vesselsCell engineeringComposite membranesCytologyEndothelial cellsFused Deposition ModelingMammalsMembranesPhosphatasesPolymeric implantsTissueTissue engineering |
|---|---|
| Engineering uncontrolled terms | ALkaline phosphataseBiofabricationCell differentiationEndothelial progenitor cellsHuman bone marrow stromal cellsIn-vivoLayer by layerVascularization |
| Engineering main heading: | Scaffolds (biology) |
| EMTREE drug terms: | alkaline phosphatasebiological markerpolylactic acidvon Willebrand factorbiomaterialpolyesterpolylactide |
| EMTREE medical terms: | angiogenesisanimal experimentanimal tissueArticlebone marrow stroma cellcell differentiationcellular distributioncoculturecontrolled studycytotoxicity assayendothelial progenitor cellendothelium cellhumanin vivo studylayer by layer bioassemblymalemembranemouseMTT assaynonhumanosteoblastphysical chemistryprotein expressiontissue engineeringvascularizationanimalartificial membranecell culturechemistrycoculturecytologydevicesendothelial progenitor cellmesenchymal stem cellmesenchymal stem cell transplantationthree dimensional printingtissue scaffoldtransplantation |
| MeSH: | AnimalsBiocompatible MaterialsCell DifferentiationCells, CulturedCoculture TechniquesEndothelial Progenitor CellsHumansMaleMembranes, ArtificialMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMiceNeovascularization, PhysiologicPolyestersPrinting, Three-DimensionalTissue EngineeringTissue Scaffolds |
Chemicals and CAS Registry Numbers:
alkaline phosphatase, 9001-78-9; polylactic acid, 26100-51-6; von Willebrand factor, 109319-16-6; polylactide, 26680-10-4;
Biocompatible Materials; Membranes, Artificial; poly(lactide); Polyesters
Funding details
| Funding sponsor | Funding number | Acronym |
|---|---|---|
| Agence Nationale de la Recherche See opportunities by ANR | ANR | |
| Université de Bordeaux | ||
| Fondation des Gueules Cassées | 57-2015 |
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1
We are grateful to Patrick Guitton (Inserm 1026) for preparation of all the figures, to Dr. Gérard Dimier, LCPO—Université de Bordeaux, for the chemical characterization of our scaffolds and to Bordeaux Imaging Center (BIC) where microscopic slides were scanned. This research was funded by a grant from “La Fondation des Gueules cassées” (57-2015) and from “l'Agence Nationale de la Recherche” (ANR Sandwich: ANR-16-CE18-0009-01). We also acknowledge the French Embassy in Serbia and French Institute in Serbia with “Campus France.”
- ISSN: 15493296
- CODEN: JBMRC
- Source Type: Journal
- Original language: English
- DOI: 10.1002/jbm.a.36769
- PubMed ID: 31376340
- Document Type: Article
- Publisher: John Wiley and Sons Inc.
Catros, S.; Biotis, INSERM U1026, Université de Bordeaux, Bordeaux Cedex, France;
© Copyright 2019 Elsevier B.V., All rights reserved.
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