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The subject of this paper is an evaluation of defined urban regions in Serbia, with an aim towards identifying their basic features, with special emphasis on the measurement of their functional polycentricity. These features include methods with following indicators: share of settled inhabitants to overall population number in the centre of urban region and surrounding area, according to the outcome; concentration level of employees in centres and structure of activities of active population performing an occupation, functional dependence of settlements in the area of the region and determining the functional polycentricity index of the urban region. Therefore, the goal of the paper is to clarify the difference between the urban regions that have developed in the area of Serbia, which are significant factors for further planning of polycentric and balanced regional development. The results of the survey show differences between urban regions. Almost half of the urban regions in Serbia have characteristics of monocentricity, while few polycentric urban regions differ in number of secondary centers. Certain differences between urban regions refer to the results related to share of the employees in primary centres in connection with the secondary centre region, if refers to a polycentric region, or in comparison to the region as a whole, if it implys to monocentric urban regions. All in all, primary differences between urban regions pertain to the degree of functional polycentrism. © 2020, Department of Geography, Tourism and Hotel Management.