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Purpose: The aim of the study was to test the efficacy, reliability, and applicability of the Kvaal and Tooth Coronal Index (TCI) age determination techniques, and then to compare them with each other, as well as with the conventional anthropological age and sex determination techniques. Methods: The analyzed material originates from the medieval necropolis of the Vinča—Belo brdo site. During the research, 60 periapical (PA) and 30 orthopantomographic (OPT) images were analyzed. On each analyzed tooth, age assessment was performed using both TCI and Kvaal techniques. The obtained values of dental estimated age were compared with age estimated by anthropological analysis, and the deviations between the estimated and chronological age were analyzed in relation to the assessment technique, type of dental radiograph, tooth group, sex, and age. Results: The mean error between TCI and the osteological method was 8.44 (SD = 7.56, Min = 0.169, Max = 36.4) and between Kvaal and the osteological method was 7.71 (SD = 5.57, Min = 0.133, Max = 26.7). The average value of age recorded by TCI method was 32.5 years and by Kvaal method was 34.7 years. There was no statistically significant difference based on the two radiographic methods, gender, individual teeth, or tooth group pairs. There was a statistically significant positive correlation between age and the error present. Conclusion: Gender determination based only on the mandible has a high correlation with the anthropological gender determination. The Kvaal method and the TCI method have proven their efficiency, reliability, and applicability. Significant correlation has been observed between dental and anthropological age and sex determination methods. © 2022, The Author(s), under exclusive licence to Springer-Verlag France SAS, part of Springer Nature.