A novel microfluidic compact disc to investigate electrochemical property changes between artificial and real salivary samples mixed with mouthwashes using electrical impedance analysis(Article)(Open Access)
- aDepartment of Biomedical Engineering, Faculty of Engineering, Universiti Malaya, Kuala Lumpur, Malaysia
- bDepartment of Biomedical Engineering, Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, Universiti Malaya, Kuala Lumpur, Malaysia
- cCentre for Printable Electronics, Universiti Malaya, Kuala Lumpur, Malaysia
- dMicrowave Research Institute, Universiti Teknologi MARA, Selangor, Shah Alam, Malaysia
- eMalaysian Research Institute on Ageing, Universiti Putra Malaysia, Selangor, Serdang, Malaysia
- fDepartment of Dental Medicine, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
- gFaculty of Technical Science, University of Novi Sad, Novi Sad, Serbia
Abstract
Diagnosing oral diseases at an early stage may lead to better preventive treatments, thus reducing treatment burden and costs. This paper introduces a systematic design of a microfluidic compact disc (CD) consisting of six unique chambers that run simultaneously from sample loading, holding, mixing and analysis. In this study, the electrochemical property changes between real saliva and artificial saliva mixed with three different types of mouthwashes (i.e. chlorhexidine-, fluoride- and essential oil (Listerine)-based mouthwashes) were investigated using electrical impedance analysis. Given the diversity and complexity of patient’s salivary samples, we investigated the electrochemical impedance property of healthy real saliva mixed with different types of mouthwashes to understand the different electrochemical property which could be a foundation for diagnosis and monitoring of oral diseases. On the other hand, electrochemical impedance property of artificial saliva, a commonly used moisturizing agent and lubricant for the treatment of xerostomia or dry mouth syndrome was also studied. The findings indicate that artificial saliva and fluoride-based mouthwash showed higher conductance values compared to real saliva and two other different types of mouthwashes. The ability of our new microfluidic CD platform to perform multiplex processes and detection of electrochemical property of different types of saliva and mouthwashes is a fundamental concept for future research on salivary theranostics using point-of-care microfluidic CD platform. © 2023 Thiha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Indexed keywords
| EMTREE drug terms: | chlorhexidineessential oilfluoridelisterinelubricating agentmouthwashsaliva substitutechlorhexidinefluoridemouth hygienesaliva substitute |
|---|---|
| EMTREE medical terms: | adultArticleconductanceelectrochemical analysisfemalehumanimmunoassayimpedancemalemicrofluidicsmouth diseasesalivaxerostomiachemistryimpedancemicrofluidicssalivaxerostomia |
| MeSH: | ChlorhexidineElectric ImpedanceFluoridesHumansMicrofluidicsMouthwashesSalivaSaliva, ArtificialXerostomia |
Chemicals and CAS Registry Numbers:
chlorhexidine, 3697-42-5, 55-56-1; fluoride, 16984-48-8; listerine, 51273-66-6;
Chlorhexidine; Fluorides; Mouthwashes; Saliva, Artificial
Funding details
| Funding sponsor | Funding number | Acronym |
|---|---|---|
| Horizon 2020 Framework Programme See opportunities by H2020 | H2020 | |
| H2020 Marie Skłodowska-Curie Actions See opportunities by MSCA | 854194,872370,IIRG002A-2022HWB | MSCA |
| Universiti Malaya | RK006-2021 | UM |
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1
This project has received funding from Universiti Malaya Partnership Grant RK006-2021, the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 872370 and 854194, and Universiti Malaya Impact Oriented Interdisciplinary Research Grant IIRG002A-2022HWB (WP1). The recipients of respective grants are as follows: Bojan Petrović, Sanja Kojić, Fatimah Ibrahim, Karunan Joseph are the recipients of the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 872370; Goran M. Stojanović and Saima Qureshi grant no. 854194. Fatimah Ibrahim, Aung Thiha, Nurul Fauzani Jamaluddin are the recipients of Universiti Malaya Partnership Grant (RK006-2021) and Universiti Malaya Impact Oriented Interdisciplinary Research Grant IIRG002A-2022HWB (WP1).
- ISSN: 19326203
- CODEN: POLNC
- Source Type: Journal
- Original language: English
- DOI: 10.1371/journal.pone.0280381
- PubMed ID: 36795661
- Document Type: Article
- Publisher: Public Library of Science
Ibrahim, F.; Department of Biomedical Engineering, Faculty of Engineering, Universiti Malaya, Kuala Lumpur, Malaysia;
Petrović, B.; Department of Dental Medicine, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia;
© Copyright 2023 Elsevier B.V., All rights reserved.
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