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In recent years, the importance of flexible and textile electronics in the field of wearable devices has continuously increased, as they are expected to replace conventional wires that exhibit limited resistance to the mechanical stress occurring in on-body applications. Wearable health devices (WHDs) can provide physiological information about various body parts and employ distributed sensor networks. Among the sensors typically integrated within WHDs, those based on the I²C communication protocol are very common and exploit signals transmitted at frequencies up to hundreds of kilohertz. Therefore, robust communication is required to guarantee a proper transmission of the signal at those frequencies. In this context, we have realized embroidered conductive threads exhibiting a lower resistance, appositely designed to replace conventional wires in a microcontroller-based wearable device employing I²C sensors. A commercial conductive thread (silver coated polyamide) was used to embroider the conductive lines on to cotton fabric. Preliminary measurements were performed to characterize the response of these materials to signals typically operated within the I²C communication protocol at different path lengths. Resistive measurements have also been performed to stimulate different environmental conditions, that is, temperature, the effect of sweating, and repeated washing cycles, also apply mechanical stress, i.e. twisting, with promising results that validate our conductive paths for digital signal communication. © 2023 The Author(s). Published by IOP Publishing Ltd.