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Objectives: The paper analyses the impact of socio-demographic characteristics of the employees in conditions of uncertainty in the organizations. Material and Methods: The study was conducted on a sample of 210 employees using the Mental Health Inventory – 38 (MHI-38), Satisfaction with life scale (SWLS), and the Center for Epidemiological Studies – Depression (CES-D). Results: The results showed that female respondents had significantly higher scores on ANX (t = 2,278, p < 0.05), while male employees had higher scores on life satisfaction (t = 2.103, p < 0.05). Older employees have a higher tendency for loss of emotional-behavioral control (F = 4.427, p < 0.05). Respondents who have satisfying living standards have also higher scores on SWLS (t = 2.257, p < 0.05). Respondents who have dissatisfying living standard have higher scores on generally positive affect (t = 3.152, p < 0.01), life satisfaction (t = 3.571, p < 0.01), psychological distress (t = 2.929, p < 0.01) and loss of emotional-behavioral control (t = 2.361, p < 0.05). Employees with different levels of educational background have similar tendencies in life satisfaction, mental health, and depressive symptoms (p > 0.05). Conclusions: The study showed that the specific socio-demographic profile of the employees is related to higher levels of mental health issues. Specifically, the mental health of female and older employees have been especially affected and disturbed by uncertain conditions. The results can be potentially used both in the terms of designing activities that support the mental health of the population, as well as in relation to the mental health of employees. © 2023 Nofer Institute of Occupational Medicine. All rights reserved.