Skip to main content
Vojnosanitetski PregledVolume 79, Issue 10, 2022, Pages 1025-1034

The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: histomorphometric and histological analysis(Article)(Open Access)

[Uticaj različitih materijala koji oblažu hidroksiapatit koštanog nosača na uspeh regeneracije koštanih defekta kalvarije zeca: histomorfometrijska i histološka analiza]

  • Milutinović-Smiljanić, S.,
  • Antonijević, D.,
  • Mićić, M.,
  • Biočanin, V.,
  • Sjerobabin, N.,
  • Petrović, B.,
  • Danilović, V.,
  • Jokanović, V.
  • View Correspondence (jump link)
  Save all to author list
  • aUniversity of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, Serbia
  • bUniversity of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, Serbia
  • cUniversity of Belgrade, Faculty of Medicine, Laboratory for Anthropology, Belgrade, Serbia
  • dUniversity of Business Academy, Faculty of Dentistry, Pančevo, Serbia
  • eUniversity Clinical Hospital Center ‟Dr. Dragiša Mišović, Belgrade, Serbia

Abstract

Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits’ calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP – Bio-Oss® (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses have been conducted in samples obtained 3, 6, and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in the HAP + PLGA group compared to other investigated materials was noticed, with no statistically significant difference between the study groups (p > 0.05). However, after 6 and 9 weeks, significant healing was observed in favor of the HAP coated with PLGA compared to other groups (p < 0.05). Within this group, greater bone healing was observed compared to the HAP + PEI and Bio-Oss® groups. Conclusion. PLGA demonstrated greater coating potential compared to PEI with respect to osteogenesis improvement in bone reconstructive surgery. © 2022 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.

Author keywords

bone regenerationhistological techniquesmaterials testingpolymersrabbits

Indexed keywords

EMTREE drug terms:acepromazinehydroxyapatiteketaminepenicillin Gpolyethyleneiminepolyglactinpolymerstreptomycinxylazine
EMTREE medical terms:animal experimentanimal modelanimal tissueArticlebone graftbone regenerationbone tissuecalvariaclinical articlefracture healinghistopathologyimplantationinflammationLeporidaematerials testingnonhumanreconstructive surgery

Chemicals and CAS Registry Numbers:

acepromazine, 61-00-7; hydroxyapatite, 1306-06-5, 51198-94-8; ketamine, 1867-66-9, 6740-88-1, 81771-21-3; penicillin G, 1406-05-9, 61-33-6; polyethyleneimine, 74913-72-7; polyglactin, 26780-50-7, 34346-01-5; streptomycin, 57-92-1; xylazine, 23076-35-9, 7361-61-7

Device tradename:

  • Leica DFC295, Leica,
  • Leitz Labor Lux S, Leitz, Germany

Manufacturers:

Device manufacturer:

Leica;

Leitz, Germany

Funding details

Funding sponsor Funding number Acronym
172026

  • 1

    This study was supported by Grant No 172026, entitled \u201CChemical and structural designing of nanomaterials for application in medicine and tissue engineering\u201D and by Grant No III 45005, entitled \u201CFunctional, functionalized, and advanced nanomaterials\u201D. They are both given by the Ministry of Education, Science, and Technological Development of the Republic of Serbia.

  • ISSN: 00428450
  • CODEN: VSPRA
  • Source Type: Journal
  • Original language: English
  • DOI: 10.2298/VSP210513072M
  • Document Type: Article
  • Publisher: Inst. Sci. inf., Univ. Defence in Belgrade

  Jokanović, V.; Institute for Nuclear Sciences “Vinča”, Laboratory for Atomic Physics, Mike Petrovica Alasa 1 Str. 2-14, Belgrade, Serbia;
© Copyright 2023 Elsevier B.V., All rights reserved.

Cited by 1 document

Ilić, V. , Biočanin, V. , Antonijević, D.
The intensity of subacute local biological effects after the implantation of ALBO-OS dental material based on hydroxyapatite and poly(lactide-co-glycolide): In vivo evaluation in rats
(2024) Biomedizinische Technik
View details of this citation
Inform me when this document is cited in Scopus:
Set citation alert Set citation feed
{"topic":{"name":"Bone Morphogenetic Protein 2; Bone Regeneration; Tissue Engineering","id":1465,"uri":"Topic/1465","prominencePercentile":92.56863,"prominencePercentileString":"92.569","overallScholarlyOutput":0},"dig":"cae58a40a144691824103c8427b089b62794db380d0a596f67c71d03373ccb57"}

SciVal Topic Prominence

Topic:
Prominence percentile: