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Sustainable development is a big global challenge for the 21st century. In recent years, a class of emerging contaminants known as microplastics (MPs) has been identified as a significant pollutant with the potential to harm ecosystems. These small plastic particles have been found in every compartment of the planet, with aquatic habitats serving as the ultimate sink. The challenge to extract MPs from different environmental matrices is a tangible and imperative issue. One of the primary specialties of research in environmental chemistry is the development of simple, rapid, low-cost, sensitive, and selective analytical methods for the extraction and identification of MPs in the environment. The present review describes the developments in MP extraction methods from complex environmental matrices. All existing methodologies (new, old, and proof-of-concept) are discussed and evaluated for their potential usefulness to extract MPs from various biotic and abiotic matrices for the sake of progress and innovation. This study concludes by addressing the current challenges and outlining future research objectives aimed at combating MP pollution. Additionally, a set of recommendations is provided to assist researchers in selecting appropriate analytical techniques for obtaining accurate results. To facilitate this process, a proposed roadmap for MP extraction is presented, considering the specific environmental compartments under investigation. By following this roadmap, researchers can enhance their understanding of MP pollution and contribute to effective mitigation strategies. © 2023 by the authors.