Laetiporus sulphureus Mushroom Enhances Cytotoxic Effect of Bifidobacterium animalis spp. lactis on HCT-116 Cells in a Co-Culture System †(Article)(Open Access)
- aInstitute for Information Technologies, University of Kragujevac, Kragujevac, 34 000, Serbia
- bDepartment for Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, 34 000, Serbia
Abstract
The study aimed to test the effect of probiotic Bifidobacterium animalis spp. lactis (BAL) on the HCT-116 cell line viability and to compare its effect with co-treatment BAL/Laetiporus sulphureus (EALS). The trypan blue staining method was used to estimate HCT-116 viability. The levels of NO2− were determined using 0.1% N-(1-naphthyl) ethylenediamine, as well as 1% sulfanilic acid. The determination of H2O2 was based on the oxidation of phenol red. Our results showed the significant cytotoxicity of the BAL on the HCT-116 cells in a co-culture system, while the BAL/EALS co-treatment further enhanced the cytotoxicity on the HCT-116 cells. We detected higher H2O2 and NO2− values in treatments with BAL, especially in the BAL/EALS co-treatment. The death of the HCT-116 cells may be due to elevated levels of H2O2 and NO2− and their products (peroxynitrites). © 2023 by the authors.
Funding details
| Funding sponsor | Funding number | Acronym |
|---|---|---|
| Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja | 175103,200122,451-03-68/2023-14/200124,451-03-47/2023-01/200122 | MPNTR |
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This research was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Agreement no. 451-03-47/2023-01/200122, 175103 and 451-03-68/2023-14/200124).
- ISSN: 26734591
- Source Type: Journal
- Original language: English
- DOI: 10.3390/ASEC2023-16608
- Document Type: Article
- Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
Arsenijević, D.; Institute for Information Technologies, University of Kragujevac, Kragujevac, Serbia;
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