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The Internet of Things (IoT) technology uses small and cost-effective sensors for various applications, such as Industrial IoT. However, these sensor nodes are powered by fixed-size batteries, which creates a trade-off between network performance and long-term sustainability. Moreover, some applications require the network to provide a certain level of service, such as a lower delay for critical data, while ensuring the operational reliability of sensor nodes. To address this energy challenge, external energy harvesting sources, such as solar and wind, offer promising and eco-friendly solutions. However, the available energy from a single energy source is insufficient to meet these requirements. This drives the utilization of a hybrid energy harvesting approach, such as the integration of solar and wind energy harvesters, to increase the amount of harvested energy. Nevertheless, to fully utilize the available energy, which is dynamic in nature, the sensor node must adapt its operation to ensure sustainable operation and enhanced network performance. Therefore, this paper proposes a hybrid energy harvesting-based energy neutral operation (ENO) medium access control (MAC) protocol, called HENO-MAC, that allows the receiver node to harvest energy from the solar-wind harvesters and adapt its duty cycle accordingly. The performance of the proposed HENO-MAC was evaluated using the latest realistic solar and wind data for two consecutive days in GreenCastalia. The simulation results demonstrate that the duty cycle mechanism of HENO-MAC effectively utilizes the harvested energy to achieve ENO and uses the available energy resources efficiently to reduce the packet delay for all packets and the highest priority packet by up to 28.5% and 27.3%, respectively, when compared with other existing MAC protocols. © 2024 IEEE.